ᱵᱷᱤᱭᱮᱴᱱᱟᱢ ᱮᱱᱴᱤᱵᱚᱰᱤᱡᱽ-ᱵᱤᱴᱭ ᱱᱳᱨᱢᱟᱱᱤ ᱱᱳᱨᱢᱟ ᱾
ᱢᱚᱰᱮᱞ: ᱕᱖᱐᱐+ ᱥᱮᱞᱮᱫ
ᱮᱵᱤᱥᱤ: ᱵᱤ ᱮᱢ ᱵᱤ ᱮᱞ ᱵᱤ ᱰᱤ᱾
ᱱᱟᱹᱢᱩᱱᱟ:
ᱤᱱᱴᱮᱜᱽᱨᱮᱥᱚᱱ
ᱥᱤᱥᱴᱮᱢ
ᱤᱱᱴᱮᱜᱽᱨᱮᱥᱚᱱ† ᱦᱟᱭᱰᱨᱳᱠᱴᱚᱢᱤ: ᱮᱱᱴᱤᱵᱟᱭᱚᱴᱤᱠ ᱨᱮ ᱜᱞᱩᱠᱚᱡᱽ ᱨᱮᱰᱤᱭᱟᱞ ᱰᱟᱭᱜᱽᱱᱚᱥᱴᱤᱠ ᱜᱞᱩᱠᱚᱡᱽ ᱨᱮ ᱮᱱᱴᱤᱵᱟᱭᱚᱴᱤᱠ ᱜᱞᱩᱠᱚᱡᱽ ᱨᱮᱭᱟᱜ ᱰᱟᱭᱜᱽᱱᱚᱥᱴᱤᱠ ᱜᱞᱩᱠᱚᱡᱽ ᱨᱮ ᱾
ᱵᱤᱜᱽᱭᱟᱯᱚᱱᱨᱚᱡᱽ:
ᱢᱮᱜᱽᱱᱤᱯᱷᱟᱭᱤᱝ:
ᱵᱤᱱᱤᱰ ᱟᱨ ᱯᱨᱚᱜᱚᱨᱟᱢᱤᱝ ᱠᱚ ᱾
ᱮᱥ ᱯᱤ ᱮᱥ ᱵᱤᱱᱤᱰ ᱞᱟᱹᱜᱤᱫ ᱑
᱑.᱑ ᱥᱚᱞᱦᱮ ᱞᱟᱹᱜᱤᱫ ᱜᱚᱲᱚ
Wᱢᱤᱫ ᱟᱨ ᱵᱟᱝ wᱡᱤᱱᱤᱥ ᱠᱚ:ᱫᱤᱥᱟᱹ ᱫᱚᱦᱚᱭᱟᱢ, ᱞᱚᱞᱚ ᱫᱚ 25-20 kg (mege) ᱞᱟᱹᱜᱤᱫ ᱵᱮᱵᱷᱟᱨ ᱦᱩᱭ ᱫᱟᱲᱮᱭᱟᱜ-ᱟ ᱟᱨ ᱚᱱᱟ ᱛᱟᱭᱚᱢ ᱨᱮ ᱢᱮᱱᱟᱜ-ᱟ ᱾ ᱚᱱᱟ ᱛᱟᱭᱚᱢ 1992 sal reak̕ gạḍi jạruṛa kạmi lạgit́ lạgit̕ lạgit́ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕ lạgit̕, (2000), ᱚᱱᱟ ᱫᱚ ᱵᱟᱝ ᱵᱟᱹᱲᱤᱡ ᱠᱟᱛᱮ, extional offical offical offical offical offical offical , exode .
᱑.᱒ ᱯᱮᱥ ᱠᱚ ᱨᱮᱭᱟᱜ ᱜᱚᱱᱚᱝ
400 kg/min, 100 mg, ᱱᱟᱹᱢᱩᱱᱟ ᱨᱮ ᱯᱮᱨᱤᱯᱷᱮᱨᱟᱞ ᱟᱨ ᱯᱷᱤᱡᱤᱥᱤᱭᱟᱱ ᱠᱚ ᱛᱟᱦᱮᱸ ᱠᱟᱱᱟ, ᱚᱱᱟ ᱛᱟᱭᱚᱢ ᱢᱤᱫ ᱫᱷᱟᱣ ᱠᱚ ᱜᱚᱡ ᱞᱮᱱᱟ ᱾ ᱥᱟᱝᱠᱨᱟᱢ ᱦᱚᱸ ᱵᱤᱱᱟᱹ ᱥᱤᱨᱡᱚᱱ ᱛᱮ ᱟᱡ ᱛᱮ ᱜᱮ ᱵᱟᱹᱲᱤᱡ ᱦᱩᱭ ᱫᱟᱲᱮᱭᱟᱜᱼᱟ ᱾
± 15 ± 10 ± 3.5 °C ᱨᱮ (ᱥᱟᱫᱷᱟᱨᱚᱱ) ᱟᱨ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱞᱟᱹᱜᱤᱫ ᱢᱮᱰᱤᱭᱚᱢ ᱨᱮ ᱰᱤ ᱮᱱ ᱮ ᱨᱮᱭᱟᱜ ᱢᱮᱠᱟᱱᱤᱡᱽᱢ ᱥᱟᱶ ᱢᱮᱥᱟ ᱠᱟᱛᱮ ᱾
ᱱᱚᱴᱥ: ᱟᱨ ᱱᱮᱜᱮᱴᱤᱵᱷ ᱠᱚᱱᱴᱨᱚᱞ ᱦᱚᱸ ᱯᱚᱡᱤᱴᱤᱵᱷ ᱞᱮᱠᱟ ᱧᱮᱞᱚᱜ ᱠᱟᱱᱟ ᱾
᱑᱓. ᱯᱮᱨᱤᱯᱷᱮᱨᱟᱞ ᱜᱞᱩᱠᱚᱡᱽ ᱠᱚ ᱾
ᱰᱤ ᱮᱱ ᱮ (᱒᱐᱑᱕) ᱫᱚ ᱱᱟᱹᱢᱩᱱᱟ (᱑:᱒) ᱜᱽᱞᱩᱠᱚᱡᱽ (᱑᱙᱕) ᱟᱨ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱨᱮ ᱢᱮᱠᱨᱚᱯᱷᱮᱡᱽ ᱨᱮᱭᱟᱜ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱞᱟᱹᱜᱤᱫ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱩᱫᱩᱜ ᱠᱮᱫᱟ᱾
᱑᱔ ᱵᱤᱱᱤᱰ ᱠᱟᱹᱢᱤᱦᱚᱨᱟ ᱾
①Take the enzyme label Microplate (according to the number of samples, can be disassembled and used several times), add 100ul of the diluted serum to the enzyme label Microplate, set two wells each for negative and positive samples, and add 100ul each for negative and positive controls to the reaction well respectively. ᱵᱮᱵᱷᱟᱨ ᱞᱟᱹᱜᱤᱫ, ᱜᱞᱩᱴᱮᱱ ᱯᱞᱮᱴ ᱨᱮ ᱓᱗ ᱴᱤᱯᱤᱡ ᱞᱟᱹᱜᱤᱫ, ᱟᱨ ᱒-᱕ ᱴᱤᱯᱤᱡ ᱞᱟᱹᱜᱤᱫ ᱥᱮᱛ ᱨᱮ ᱫᱚᱦᱚ ᱠᱟᱛᱮ, ᱥᱮᱢᱯᱚᱞ ᱠᱚ ᱵᱮᱵᱷᱟᱨ ᱢᱮ᱾
30 μm, glock lạgit̕ lạgit̕ lạgit̕ lạgit̕ gạḍi kạmi lạgit̕ lạgit̕ lạḍi kạmi lạgit̕ lạgit̕ lạgit̕ lạḍi jạruṛ ge kạmi lạgit̕ lạḍi ḍạr kạmi lạgit̕ lạḍi kana, ᱚᱱᱟ ᱫᱚ ᱯᱞᱟᱴᱤᱱᱟᱢ ᱠᱟᱱᱟ᱾ ᱪᱮᱛᱟᱱ ᱟᱨ ᱞᱟᱛᱟᱨ ᱨᱮ ᱕ ᱫᱷᱟᱣ ᱪᱷᱟᱯᱟ ᱞᱟᱹᱜᱤᱫ, ᱢᱤᱫ ᱚᱠᱛᱚ ᱨᱮ ᱢᱤᱫ ᱫᱷᱟᱣ ᱫᱚᱦᱚ ᱠᱟᱜ ᱢᱮ ᱾
50 μl 100 mL ᱨᱮ 30 μm, ᱟᱨ 1 μm, ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱞᱟᱹᱜᱤᱫ ᱢᱤᱫ ᱱᱟᱹᱢᱩᱱᱟ ᱞᱟᱹᱜᱤᱫ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱫᱚ ᱱᱟᱹᱢᱩᱱᱟ ᱞᱟᱹᱜᱤᱫ ᱢᱤᱫ ᱫᱷᱟᱣ ᱜᱮ ᱾
30 μl imaption lạgit̕ gạḍi jạruṛ ge kạmi lạgit̕, equion lạgit̕ lạgit̕ lạḍi jạruṛ ge lạgit̕ lạḍi jạruṛ ge kạmi lạgit̕ lạḍi kạmi lạgit́, ᱚᱱᱟ ᱫᱚ ᱯᱞᱟᱴᱤᱱᱟᱢ ᱠᱚ ᱫᱚᱦᱚ ᱠᱟᱜ-ᱟ ᱾ ᱪᱮᱛᱟᱱ ᱟᱨ ᱞᱟᱛᱟᱨ ᱨᱮ ᱕ ᱫᱷᱟᱣ ᱪᱷᱟᱯᱟ ᱞᱟᱹᱜᱤᱫ, ᱢᱤᱫ ᱚᱠᱛᱚ ᱨᱮ ᱢᱤᱫ ᱫᱷᱟᱣ ᱫᱚᱦᱚ ᱠᱟᱜ ᱢᱮ ᱾
10 μl ᱠᱷᱚᱱ 100 μm ᱦᱟᱹᱵᱤᱡ, ᱟᱨ 30 μl ᱠᱷᱚᱱ 1 μl ᱫᱚ ᱥᱟᱱᱟᱢ ᱠᱷᱚᱱ ᱵᱮᱥ ᱞᱚᱞᱚ ᱛᱮ ᱢᱤᱫ ᱪᱟᱸᱫᱚ ᱨᱮ ᱢᱤᱫ ᱥᱟᱶᱛᱮ ᱢᱮᱥᱟᱞ ᱞᱟᱹᱜᱤᱫ ᱥᱟᱯᱲᱟᱣ ᱠᱟᱱᱟ ᱾
50 μm ᱟᱨ 5 μm ᱦᱟᱹᱵᱤᱡ, ᱥᱟᱱᱟᱢ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱫᱚ ᱠᱚᱢ ᱥᱮ ᱠᱚᱢ ᱢᱤᱫ ᱥᱚᱢᱟᱫᱷᱟᱱ ᱠᱟᱱᱟ ᱾
ᱮᱥ ᱴᱤ ᱒ ᱨᱮᱯᱞᱤᱠᱮᱥᱚᱱ ᱯᱟᱲᱦᱟᱣ
ᱥᱟᱱᱟᱢ ᱡᱤᱱᱤᱥ ᱨᱮ ᱙᱖-᱔᱐᱕-ᱢᱤᱢᱤᱴᱚᱨ ᱢᱟᱭᱠᱨᱚᱥᱠᱳᱯᱤᱠ ᱯᱟᱲᱦᱟᱣ ᱠᱚ ᱛᱟᱦᱮᱸ ᱠᱟᱱᱟ ᱾
᱒.᱑ ᱜᱚᱱᱚᱝ
450 × g ᱨᱮᱭᱟᱜ ᱜᱩᱱ ᱠᱚ ᱨᱮᱭᱟᱜ ᱡᱷᱩᱠᱤ ᱠᱚ ᱛᱟᱦᱮᱸ ᱠᱟᱱᱟ ᱾<0.1 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥3, the experimental results were valid. Otherwise, the test should be repeated.
᱒.᱒ ᱠᱟᱨᱴᱨᱤᱡᱽ ᱡᱚᱠᱷᱟ
ᱥᱟᱱᱟᱢ ᱵᱤᱱᱤᱰ ᱞᱟᱹᱜᱤᱫ, g/ms ᱨᱮᱭᱟᱜ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱫᱚ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱟᱨ ᱱᱟᱹᱢᱩᱱᱟ ᱠᱚ ᱨᱮᱭᱟᱜ ᱵᱮᱵᱷᱟᱨ ᱠᱚ ᱨᱮ ᱴᱮᱦᱟᱱᱰ ᱢᱮᱱᱟᱜᱼᱟ:
᱒᱓ ᱥᱮᱨᱢᱟ ᱵᱷᱤᱛᱨᱤ ᱨᱮ ᱾
❤️/<0.25, it is judged as negative.
ᱮᱴᱟᱜ ᱠᱚ ᱫᱚ ᱯᱤᱯᱤᱥᱤ ᱠᱷᱚᱱ ᱕/᱓ ᱠᱷᱚᱱ ᱵᱟᱹᱲᱛᱤ ᱠᱚ ᱞᱟᱹᱠᱛᱤᱭᱟ, ᱡᱩᱫᱤ ᱵᱟᱝ ᱠᱷᱟᱱ ᱫᱚ ᱵᱟᱝ ᱾
ᱡᱟᱦᱟᱸ ᱫᱚ €᱒᱕᱐ ᱠᱷᱚᱱ € ᱦᱟᱹᱵᱤᱡ ᱟᱨ ᱵᱟᱝᱠᱷᱟᱱ ᱥᱤ-᱐ ᱾<0.35, it is judged as suspicious, and suggest to repeat sampling and test one week later.
ᱦᱚᱜᱽᱥ: ᱮᱨᱚᱵᱤᱠ ᱮᱱᱴᱤᱵᱟᱭᱚᱴᱤᱠ, ᱥᱤᱜᱱᱟᱞ, ᱮᱱᱴᱤᱵᱟᱭᱚᱴᱤᱠ ᱜᱞᱩᱠᱚᱡᱽ ᱜᱞᱩᱠᱚᱡᱽ, ᱪᱟᱭᱱᱟ ᱨᱮ ᱮᱱᱴᱤᱵᱟᱭᱚᱴᱤᱠ ᱜᱞᱩᱠᱚᱡᱽ ᱜᱞᱩᱠᱚᱡᱽ ᱜᱞᱩᱠᱚᱡᱽ ᱜᱞᱩᱠᱚᱡᱽ ᱜᱞᱩᱠᱚᱡᱽ ᱾
